Aseptic Techniques: Plate Streaking Quiz

1. The purpose of streaking a plate is to obtain
large quantities of bacteria
bacteria in pure culture

2. When streaking a plate, primarily
flex and extend the fingers
move from the wrist
move your whole arm

3. Plates should be incubated
agar side up
agar side down
it doesn't matter

4. When streaking a plate, you should go back over the last area
never
only once
2 times
3-4 times
8-10 times

5. When streaking a plate, the lid should be
placed on the bench top, top side up
placed on the bench top, inside surface up
should be held in the same hand as the plate
should be held in the opposite hand from the plate

6. When streaking a plate, the loop should be flamed
At the beginning and end
At the beginning and end and between the first 2 zones
At the beginning and end and between the second and third zone
At the beginning and end and between each zone
never

7. George streaks a plate of medium from a broth culture.  After incubation, he sees confluent growth in all sectors.  Which of the following errors could NOT explain this result?
He forgot to pick a colony from the plate
He forgot to flame the loop between sectors
He picked additional culture from the plate after the first sector
He crossed over the previous streak too many times at the beginning of a new sector.

   

On To Broth Transfer