Microbiology Skills: Pipetting

Types of Pipettes

Glass pipettes are calibrated cylinders that allow small volumes (usually 0.1 ml to 10 ml) to be accurately dispensed. Although they have been replaced to some extent by automatic pipettors with plastic disposable tips, they are still used routinely.

Pipette Aids

Link to Scienceware instructions in PDF format for the [Fast Release Pipette Pump]

Aseptic Pipetting

  • Tilt a can of pipettes so that the tops of the pipettes are at the top of the can.
  • Set the can on the laboratory bench so that the top of the can extends off the edge of the bench.
  • Remove the lid from the can and set the lid on the countertop.
  • Without removing it from the can, pick up a pipette with your non-dominant hand. Use your dominant hand to place a pipette aid on the pipette.
  • Slide the pipette from the can, lifting it to avoid dragging the bottom of the pipette across the contaminated surfaces of the other pipettes.
  • Use the little finger of the hand holding the pipette to remove the lid from the container containing your sample.
    Touch the tip of the pipette to the side of the container.
  • Measure sample into the container.
  • Place the pipette into a discard container and remove the pipette aid.

On To Plating