Aseptic Techniques: Pipette Transfer

  • Tilt a can of pipettes so that the tops of the pipettes are at the top of the can.
  • Set the can on the laboratory bench so that the top of the can extends off the edge of the bench.
  • Remove the lid from the can and set the lid on the countertop.
  • Without removing it from the can, pick up a pipette with your non-dominant hand. Use your dominant hand to place a pipette aid on the pipette.
  • Slide the pipette from the can, lifting it to avoid dragging the bottom of the pipette across the contaminated surfaces of the other pipettes.
  • Use the little finger of the hand holding the pipette to remove the lid from the container containing your sample.
  • Touch the tip of the pipette to the side of the container.
  • Measure sample into the container.
  • Place the pipette into a discard container and remove the pipette aid.

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