Broth Transfer Technique

  • Loosen the test tube cap, if necessary, but do not remove it.
  • Hold the loop in your dominant hand and flame-sterilize1 it. Allow the loop to cool a few seconds.
  • Pick up the test tube in your non-dominant hand. Use the little finger of the hand holding the loop to remove the cap from the test tube. Do not set the cap down.
  • Insert the loop into the tube and pick up a loopful of culture.
  • Remove the loop and carefully replace the cap on the tube.
  • Return the tube to the rack.
  • Pick up the tube you wish to inoculate.
  • Remove the lid as before, again holding the lid in the little finger of the hand holding the loop.
  • Slide the loop into the tube. (You do not need to rattle the loop to shake off the bacteria.)
  • Remove the loop and carefully replace the cap on the tube.
  • Return the tube to the rack.
  • Flame the loop and set it down.

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