Aseptic Techniques: Broth Transfer Quiz

1. Which finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer?
little finger of the dominant hand (hand holding the loop)
little finger of the non-dominant hand (hand holding the sample)
forefinger of the dominant hand (hand holding the loop)
thumb and forefinger of the non-dominant hand (hand holding the sample)

2. The bacteriology loop should be sterilized in what part of the Bunsen burner flame?
in the bright blue cone
just above the bright blue cone
above the flame
at the base of the flame

3. The loop should be held
straight up and down
at a very slight angle
at a fairly steep angle
parallel to the work bench

   

On to Pipette Transfer